Long-read sequencing, metagenomics and the microbiome

Talking Techniques - En podkast av BioTechniques

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In this episode, sponsored by Zymo Research, we explore the microbiome and how long-read sequencing techniques are changing our approach to the field and the exciting discoveries that they have led to. To do this we speak to Kris Locken, Molecular Biologist in the Research & Development department of Zymo Research (CA, USA), and Jeremy Wilkinson, Global Marketing Specialist for Microbial Genomics at PacBio (CA, USA).Find out how microbes compare to nuts and what this means for the challenges of metagenomic sample preparation, how long reads can stack up vs short reads for metagenome assembly and much more, all in this latest episode of Talking TechniquesContents:Intro: 00:00-01:55What is metagenomics and why is it important for microbial studies? 01:55-03:55How has long-read sequencing impacted metagenomics? 03:55-06:05Long-read vs short-read sequencing workflows: 06:05-07:15Addressing barriers to long-read sequencing: 07:15-08:45Sample preparation for long-read sequencing: 08:45-12:20Development of long-read sequencing to improve accuracy and capabilities: 12:20-14:40Best practice for assembly and analysis: 14:40-17:40Exciting examples of the benefits of long-read sequencing: 17:40-20:00What would you wish for to improve the ability long-read sequencing in microbiomics: 20:00-21:40 Hosted on Acast. See acast.com/privacy for more information.

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